Rapid diagnosis of coccidioidomycosis by nested PCR assay of sputum
نویسندگان
چکیده
منابع مشابه
Comparison of Betanodavirus diagnosis quality in Grouper and Sea bass by Rapid Diagnosis Kit, histopathology and Nested-PCR methods
Betanodavirus is the leading cause of viral mortality in fish worldwide and a major barrier to the spread of fisheries and aquaculture. Due to the increase in aquaculture in the Persian Gulf and the occurrence of some disease symptoms in farmed and wild fish in the region, screening of Betanodavirus-sensitive fish became important. Therefore, to prevent economic losses and reduce aquatic reserv...
متن کاملDiagnosis and monitoring of murine histoplasmosis by a nested PCR assay.
A newly developed nested PCR assay was applied to murine models of histoplasmosis. ICR and BALB/c mice were intravenously infected with Histoplasma capsulatum and sacrificed up to 29 days later. Samples of blood, spleen, and lung homogenates were cultured and examined by the PCR assay. In the ICR mouse model, 265 of 319 organ samples showed concordant results. With 7 samples, the culture was po...
متن کاملDiagnosis of Barmah Forest Virus Infection by a Nested Real-Time SYBR Green RT-PCR Assay
Barmah Forest virus (BFV) is a mosquito borne (+) ssRNA alphavirus found only in Australia. It causes rash, myalgia and arthralgia in humans and is usually diagnosed serologically. We developed a real-time PCR assay to detect BFV in an effort to improve diagnosis early in the course of infection. The limit of detection was 16 genome equivalents with a specificity of 100%. Fifty five serum sampl...
متن کاملDiagnosis of Bartonella endocarditis by a real-time nested PCR assay using serum.
Bartonella endocarditis is a severe disease for which blood cultures frequently remain negative. We tested three PCR assays by using specimens of serum sampled early during the disease from 43 patients diagnosed in our laboratory as having Bartonella endocarditis on the basis of serological, culture, and/or valvular molecular detection. We tested a two-step nested PCR (TSN-PCR), a one-step nest...
متن کاملRapid diagnosis of leptospirosis by multiplex PCR.
BACKGROUND Traditionally, the most common diagnostic approach used for diagnosing leptospirosis was the demonstration of immune-seroconversion in acute and convalescent patient serum samples. Recently, a variety of molecular techniques, including conventional and real-time polymerase chain reaction (PCR), have been developed for the specific detection of pathogenic bacteria from the genus Lepto...
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ژورنال
عنوان ژورنال: Clinical Microbiology and Infection
سال: 2007
ISSN: 1198-743X
DOI: 10.1111/j.1469-0691.2006.01679.x